Synthetic biology aims to solve challenges like pollution and gene therapies but needs improved DNA synthesis technology for commercial applications. Traditional phosphoramidite synthesis creates DNA up to 350 nucleotides but struggles with complex sequences. Enzymatic synthesis, particularly methods utilizing terminal deoxynucleotidyl transferase (TdT), offers a promising alternative. Companies like DNA Script and Ansa Biotechnologies are developing techniques that leverage TdT for efficient and template-free DNA synthesis, allowing for longer and more complex DNA fragments, overcoming limitations of previous methods.
Enzymatic synthesis inspired by cellular DNA replication can produce custom DNAs without templates. Terminal deoxynucleotidyl transferase (TdT) joins nucleotides together efficiently.
DNA Script uses TdT with modified nucleotides to control elongation. Each base is sequentially added after protecting groups are removed, enabling the synthesis of longer DNA fragments.
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